Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | stimulates | Escherichia coli | |
Cobalamin | stimulates | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxyqueuosine34 in tRNA + reduced acceptor | Escherichia coli | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced acceptor | Bacillus subtilis | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced acceptor | Bacillus subtilis 168 | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P97030 | - |
- |
Bacillus subtilis 168 | P97030 | - |
- |
Escherichia coli | P39288 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Escherichia coli | queuosine34 in tRNA + acceptor + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis | queuosine34 in tRNA + acceptor + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis 168 | queuosine34 in tRNA + acceptor + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced methyl viologen | conversion of epoxyqueuosine to queuoseine is carried out using either a 17-mer oQ-containing RNA stem loop | Escherichia coli | queuosine34 in tRNA + methyl viologen + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced methyl viologen | the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain | Bacillus subtilis | queuosine34 in tRNA + methyl viologen + H2O | - |
? | |
epoxyqueuosine34 in tRNA + reduced methyl viologen | the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain | Bacillus subtilis 168 | queuosine34 in tRNA + methyl viologen + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oQ reductase | - |
Escherichia coli |
oQ reductase | - |
Bacillus subtilis |
QueG | - |
Escherichia coli |
QueG | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
7.2 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Escherichia coli |
metabolism | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis |